Bacterial Artificial Chromosomes: Volume 2 Functional Studies


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Login in here. The Keio Journal of Medicine. Journal home Advance online publication Journal issue About the journal. The development and applications of the bacterial artificial chromosome cloning system. Published: received: - Released: June 16, accepted: - [Advance Publication] Released: - corrected: -. Article overview. References Efficient library screening is crucial for all applications of the library.

PCR screening is a popular method because of its reliability, speed, and efficiency, with high specificity. The PCR numbers for positive clones detected by different methods are shown in Table 3. The two-step method can save time in identifying positive clones, given the high PCR throughout and reduced costs of PCR today.

Although it is unclear whether the target gene is covered by the selected BAC clones, this positive clone identification is an important step in arriving at the resistance genes through this method. In the next step, positive clones can be lined up to form an initial contig, which is the basis of chromosome walking. Theoretical and Applied Genetics Plant Molecular Biology Reporter Acta Genetica Sinica Acta Horticulturae Sinica Hereditas Journal of Biomedicine and Biotechnology Molecular Plant-Microbe Interactions Genome Journal of Maize Science Science BMC Plant Biology Journal of Genetics and Genomics Scientia Agricultura Sinica Zhang H, Choi S, Woo S, Li Z and Wing R Construction and characterization of two rice bacterial artificial chromosome libraries from the parents of a permanent recombinant inbred mapping population.

Molecular Breeding 2: Molecular Breeding Molecular Genetics and Genomics This is an open-access article distributed under the terms of the Creative Commons Attribution License. Services on Demand Journal. Abstract Zea mays L. Detection of positive clones by using primers linked to the resistance genes Primers for positive clone detection According to the results of previous studies Chen et al.

Quality testing of the BAC library Test of insert size and empty clone rate A total of clones were selected from the library, and DNA was isolated from the clones to study the insert size distribution and to estimate both the average insert size in the library and the empty clone rate. Received: November 17, ; Accepted: August 12, E-mail: snakepy Narayanan, "Intact recombineering of highly repetitive DNA requires reduced induction of recombination enzymes and improved host viability," Analytical Biochemistry, vol.

Zhang, J. Muyrers, G. Testa, and A. Ellis, D.

DNA sequencing (BAC to BAC sequencing)

Yu, T. DiTizio, and D. Court, "High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides," Proceedings of the National Academy of Sciences of the United States of America, vol. Yu, J. Sawitzke, H. Ellis, and D. Court, "Recombineering with overlapping single-stranded DNA oligonucleotides: testing a recombination intermediate," Proceedings of the National Academy of Sciences of the United States of America, vol.

Muyrers, Y.

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Zhang, G. Karu, Y. Sakaki, H. Echols, and S. Friedman and J. Datta, N. Costantino, X. Zhou, and D. Court, "Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages," Proceedings of the National Academy of Sciences of the United States of America, vol. Imam, G. Patrinos, M. Jamsai, M. Orford, M. Nefedov, S. Fucharoen, R.

Williamson, and P. Warming, N. Costantino, D. Court, N. Jenkins, and N. Wong, V. Ng, M. Lin, H. Kung, D. Chan, and J. Kouprina and V. Larionov, "TAR cloning: insights into gene function, long-range haplotypes and genome structure and evolution," Nature Reviews Genetics, vol. Mullins, N. Kotelevtseva, A. Boyd, and J. Mullins, "Efficient Cre-lox linearisation of BACs: applications to physical mapping and generation of transgenic animals," Nucleic Acids Research, vol. Mejia and Z. Hoess and K.

Abremski, "Mechanism of strand cleavage and exchange in the Cre-lox site-specific recombination system," Journal of Molecular Biology, vol. McLeod, S. Craft, and J.

Bacterial Artificial Chromosomes: Volume 2: Functional Studies - PDF Free Download

Broach, "Identification of the crossover site during FLP-mediated recombination in the Saccharomyces cerevisiae plasmid 2 microns circle," Molecular and Cellular Biology, vol. Sauer, "Site-specific recombination: developments and applications," Current Opinion in Biotechnology, vol. O'Gorman, D. Fox, and G.

Wahl, "Recombinase-mediated gene activation and site-specific integration in mammalian cells," Science, vol. Fu, M. Teucher, K. Anastassiadis, W. Skarnes, and A. Stewart, "A recombineering pipeline to make conditional targeting constructs," Methods in Enzymology, vol. Stahl and M. Durfee, R.

1. Introduction

Nelson, S. Baldwin et al. O'Connor, M. Peifer, and W. Messerle, I. Crnkovic, W. Hammerschmidt, H. Ziegler, and U. Koszinowski, "Cloning and mutagenesis ofa herpesvirus genome as an infectious bacterial artificial chromosome," Proceedings of the National Academy of Sciences of the United States ofAmerica, vol. Yang, P.

Model, and N. Heintz, "Homologous recombination based modification in Esherichia coli and germline transmission in transgenic mice of a bacterial artificial chrom-some," Nature Biotechnology, vol. Degryse, "In vivo intermolecular recombination in Escherichia coli: application to plasmid constructions," Gene, vol. Chartier, E. Degryse, M. Gantzer, A. Dieterle, A. Pavirani, and M. Mehtali, "Efficient generation of recombinant aden-ovirus vectors by homologous recombination in Escherichia coli," Journal of Virology, vol. He, S.

Zhou, L. Da Costa, J. Yu, K. Kinzler, and B. Vogelstein, "A simplified system for generating recombinant adenoviruses," Proceedings of the National Academy of Sciences. Bubeck, M. Winkler, and W. Bautsch, "Rapid cloning by homologous recombination in vivo," Nucleic Acids Research, vol. Kaiser and N.


  1. When Raccoons Fall through Your Ceiling: The Handbook for Coexisting with Wildlife;
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Murray, "Physical characterisation of the 'Rac prophage' in E. Oliner, K. Murray, "On the nature of sbcA mutations in E. Barbour, H. Nagaishi, A. Templin, and A. Clark, "Biochemical and genetic studies of recombination proficiency in Escherichia coli. Kiel, J. Vossen, and G. Venema, "A general method for the construction of Escherichia coli mutants by homologous recombination and plasmid segregation," Molecular and General Genetics, vol.

Murphy, K. Campellone, and A. Yu, H. Ellis, E. Lee, N. Jenkins, N. Copeland, and D. Court, "An efficient recombination system for chromosome engineering in Escherichia coli," Proceedings of the National Academy of Sciences of the United States of America, vol. Kawaguchi and S. Kuramitsu, "Homologous ligation: a novel cloning method," Trends in Genetics, vol. Sopher and A. La Spada, "Efficient recombination-based methods for bacterial artificial chromosome fusion and mutagenesis," Gene, vol.

Gong, C. Zheng, M. Doughty et al. Gong and X. Wang, M. Sarov, J. Rientjes et al. Muyrers, J. Rientjes, and F. Yang and B. Seed, "Site-specific gene targeting in mouse embryonic stem cells with intact bacterial artificial chromosomes," Nature Biotechnology, vol. Song, S. Chung, and Y. Kotzamanis and C. Crouzet, L. Naudin, C. Orsini et al. Domi and B. Moss, "Engineering of a vaccinia virus bacterial artificial chromosome in Escherichia coli by bacteriophage X-based recombination," Nature Methods, vol.

Wussow, H.

Bacterial Artificial Chromosomes Volume 2: Functional Studies Shaying Zhao

Fickenscher, and B. Tischer, "Red-mediated transposition and final release of the mini-F vector of a cloned infectious herpesvirus genome," PloS One, vol. Westenberg, H. Soedling, D. Mann, L. Nicholson, and C.

Bacterial Artificial Chromosomes: Volume 2 Functional Studies Bacterial Artificial Chromosomes: Volume 2 Functional Studies
Bacterial Artificial Chromosomes: Volume 2 Functional Studies Bacterial Artificial Chromosomes: Volume 2 Functional Studies
Bacterial Artificial Chromosomes: Volume 2 Functional Studies Bacterial Artificial Chromosomes: Volume 2 Functional Studies
Bacterial Artificial Chromosomes: Volume 2 Functional Studies Bacterial Artificial Chromosomes: Volume 2 Functional Studies
Bacterial Artificial Chromosomes: Volume 2 Functional Studies Bacterial Artificial Chromosomes: Volume 2 Functional Studies
Bacterial Artificial Chromosomes: Volume 2 Functional Studies Bacterial Artificial Chromosomes: Volume 2 Functional Studies
Bacterial Artificial Chromosomes: Volume 2 Functional Studies Bacterial Artificial Chromosomes: Volume 2 Functional Studies

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