Login in here. The Keio Journal of Medicine. Journal home Advance online publication Journal issue About the journal. The development and applications of the bacterial artificial chromosome cloning system. Published: received: - Released: June 16, accepted: - [Advance Publication] Released: - corrected: -. Article overview. References Efficient library screening is crucial for all applications of the library.
PCR screening is a popular method because of its reliability, speed, and efficiency, with high specificity. The PCR numbers for positive clones detected by different methods are shown in Table 3. The two-step method can save time in identifying positive clones, given the high PCR throughout and reduced costs of PCR today.
Although it is unclear whether the target gene is covered by the selected BAC clones, this positive clone identification is an important step in arriving at the resistance genes through this method. In the next step, positive clones can be lined up to form an initial contig, which is the basis of chromosome walking. Theoretical and Applied Genetics Plant Molecular Biology Reporter Acta Genetica Sinica Acta Horticulturae Sinica Hereditas Journal of Biomedicine and Biotechnology Molecular Plant-Microbe Interactions Genome Journal of Maize Science Science BMC Plant Biology Journal of Genetics and Genomics Scientia Agricultura Sinica Zhang H, Choi S, Woo S, Li Z and Wing R Construction and characterization of two rice bacterial artificial chromosome libraries from the parents of a permanent recombinant inbred mapping population.
Molecular Breeding 2: Molecular Breeding Molecular Genetics and Genomics This is an open-access article distributed under the terms of the Creative Commons Attribution License. Services on Demand Journal. Abstract Zea mays L. Detection of positive clones by using primers linked to the resistance genes Primers for positive clone detection According to the results of previous studies Chen et al.
Quality testing of the BAC library Test of insert size and empty clone rate A total of clones were selected from the library, and DNA was isolated from the clones to study the insert size distribution and to estimate both the average insert size in the library and the empty clone rate. Received: November 17, ; Accepted: August 12, E-mail: snakepy Narayanan, "Intact recombineering of highly repetitive DNA requires reduced induction of recombination enzymes and improved host viability," Analytical Biochemistry, vol.
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Bacterial Artificial Chromosomes: Volume 2: Functional Studies - PDF Free Download
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Bacterial Artificial Chromosomes Volume 2: Functional Studies Shaying Zhao
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